We utilize a wide array of imaging techniques such as widefield microscopy, confocal microscopy, and total internal reflection fluorescence microscopy. We have also developed several ensemble and single molecule imaging techniques that we use to answer questions in the context of our research interests.

Single Molecule Techniques

Our group develops cutting edge single molecule techniques. We have recently developed a new method to monitor changes in protein assembly that takes place in the central nervous system of a live animal by extracting nanoscale vesicles that encapsulate the protein of interest.

We use nanofabricated devices such as zero-mode waveguides to image membrane proteins in live cells. We have applied this technique to monitor single ligand-receptor binding events.

Multi-Photon Microscopy

We use mutliphoton microscopy for intravital imaging through cranial windows to help better understand the mechanism of nicotine addiction.

Atomic Force Microscopy

We have access to a combined confocal and atomic force microscope. We use this instrument to better understand the structural rearrangement of cells in response to environmental changes.