Publications

  1. Qianye Yin, Surya P. Aryal, Yongwook Song, Xu Fu, Christopher I. Richards. Quantitative Single-Molecule Analysis of Ryanodine Receptor 2 Subunit Assembly in Cardiac and Neuronal Tissues. Analytical Chemistry. 2024. https://pubs.acs.org/doi/10.1021/acs.analchem.4c03314

  2. Khaga R. Neupane, Surya P. Aryal, Brock T. Harvey, Geraldine San Ramon, Byeong Chun, J. Robert McCorkle, Jill M. Kolesar, Peter M. Kekenes-Huskey, Christopher I. Richards. Organelle Specific Macrophage Engineered Vesicles Differentially Reprogram Macrophage Polarization. Advanced Healthcare Materials. 2024. https://doi.org/10.1002/adhm.202401906

  3. Mohit Kumar, Jack Keady, Surya P. Aryal, Marissa Hessing, Christopher I. Richards, Jill R. Turner. The Role of Microglia in Sex- and Region-Specific Blood-Brain Barrier Integrity During Nicotine Withdrawal. Biological Psychiatry Global Open Science. 2023. https://doi.org/10.1016/j.bpsgos.2023.08.019

  4. Jourdan E. Lakes, Xu Fu, Brock Harvey, Khaga R. Neupane, Surya P. Aryal, Jessica L. Ferrell, Michael D. Flythe, Christopher I. Richards. Impact of nicotine and cotinine on macrophage inflammatory plasticity via vesicular modifications in gastrointestinal bacteria. Anaerobe, 2023. 102787. https://doi.org/10.1016/j.anaerobe.2023.102787

  5. Neupane, K. R., Ramon, G. S., Harvey, B., Chun, B., Aryal, S. P., Masud, A. A., McCorkle, J. R., Kolesar, J. M., Kekenes-Huskey, P. M., Richards, C. I., Programming Cell-Derived Vesicles with Enhanced Immunomodulatory Properties. Adv. Healthcare Mater. 2023, 2301163. https://doi.org/10.1002/adhm.202301163

  6. Xiaojin Wang, Mikkel B. Liisberg, Georgia L. Vonlehmden, Xu Fu, Cecilia Cerretani, Lan Li, Lance A. Johnson, Tom Vosch, and Christopher I. Richards. DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy. ACS Nano. 2023. https://doi.org/10.1021/acsnano.3c04489

  7. Ahmed Ismaeel, Douglas W. Van Pelt, Zachary R. Hettinger, Xu Fu, Christopher I. Richards, Timothy A. Butterfield, Jonathan J. Petrocelli, Ivan J. Vechetti, Amy L. Confides, Micah J. Drummond & Esther E. Dupont-Versteegden. Extracellular vesicle distribution and localization in skeletal muscle at rest and following disuse atrophy. Skeletal Muscle, 13, 6. 2023. https://doi.org/10.1186/s13395-023-00315-1

  8. Byeong J. Chun, Surya P. Aryal, Peter Varughese, Bin Sun, Joshua A. Bruno, Chris I. Richards, Adam D. Bachstetter and Peter M. Kekenes-Huskey. Purinoreceptors and ectonucleotidases control ATP-induced calcium waveforms and calcium-dependent responses in microglia: Roles of P2 receptors and CD39 in ATP-stimulated microglia. Frontiers in Physiology, Computational Physiology and Medicine. 2023. https://doi.org/10.3389/fphys.2022.1037417

  9. David Schweer, Namrata Anand, Abigail Anderson, J. Robert McCorkle, Khaga Neupane, Alexandra N. Nail, Brock Harvey, Kristen S Hill, Frederick Ueland, Christopher Richards, Jill Kolesar. Human Macrophage-Engineered Vesicles for Utilization in Ovarian Cancer Treatment. Frontiers in Oncology, Molecular and Cellular Oncology. 2022. https://doi.org/10.3389/fonc.2022.1042730

  10. Brock T. Harvey, Xu Fu, Lan Li, Khaga R. Neupane, Namrata Anand, Jill M. Kolesar, and Christopher I. Richards. Dendritic Cell Membrane-Derived Nanovesicles for Targeted T Cell Activation. ACS Omega. 2022. https://doi.org/10.1021/acsomega.2c04420

  11. Surya P. Aryal,Mengfan Xia,Pavel I. Ortinski,Christopher I. Richards, Study of Calcium Signaling in Astrocytes with a Novel Endoplasmic Reticulum–Targeted GCaMP Sensor. Current PRotocols. 2022. https://doi.org/10.1002/cpz1.491

  12. Messina TC, Srijanto BR, Collier CP, Kravchenko II, Richards CI. Gold Ion Beam Milled Gold Zero-Mode Waveguides. Nanomaterials. 2022; 12(10):1755. https://doi.org/10.3390/nano12101755

  13. Schweer, D.; McAtee, A.; Neupane, K.; Richards, C.; Ueland, F.; Kolesar, J. Tumor-Associated Macrophages and Ovarian Cancer: Implications for Therapy. Cancers 2022, 14, 2220. https://www.mdpi.com/2072-6694/14/9/2220

  14. Surya P. Aryal, Mengfan Xia, Ebubechi Adindu, Caroline Davis, Pavel I. Ortinski, and Christopher I. Richards (2022). ER-GCaMP6f: An Endoplasmic Reticulum-Targeted Genetic Probe to Measure Calcium Activity in Astrocytic Processes. Analytical Chemistry 2022 94 (4), 2099-2108 https://doi.org/10.1021/acs.analchem.1c04321

  15. Dongseok Kim, Rosemary L Calabro, Abdullah A Masud, Nadeesha L Kothalawala, Minsu Gu, Seung-Yeon Kwak, Won-Joon Son, Kyu Young Hwang, Hyeonho Choi, Christopher I Richards, Doo Young Kim, Byeong-Su Kim (2021). Exploring the Role of Surface States in Emissive Carbon Nanodots: Analysis at a Single-Particle Level. Chemistry: An Asian Journal doi.org/10.1002/asia.202101087

  16. Aryal, S. P., Neupane, K. R., Masud, A. A., & Richards, C. I. (2021). Characterization of astrocyte morphology and function using a fast and reliable tissue clearing technique. Current Protocols, 1, e279. DOI: 10.1002/cpz1.279

  17. Hui Peng, Brock T. Harvey, Christopher I. Richards, and Kimberly Nixon. Neuron-Derived Extracellular Vesicles Modulate Microglia Activation and Function. Biology: In Press. DOI: 10.3390/biology10100948, (2021)

  18. Aryal, S. P., Fu, X., Masud, A. A., Neupane, K. R. and Richards, C. I. (2021). Single-Molecule Studies of Membrane Receptors from Brain Region Specific Nanovesicles. Bio-protocol 11(10): e4018. DOI: 10.21769/BioProtoc.4018.

  19. Surya P. Aryal, Xu Fu, Joree N. Sandin, Khaga R. Neupane, Jourdan E. Lakes, Martha E. Grady, and Christopher I. Richards. Nicotine induces morphological and functional changes in astrocytes via nicotinic receptor activity. Glia, 2021, doi.org/10.1002/glia.24011

  20. Abdullah Al Masud, S. M. Nayeem Arefin, Fatema Fairooz, Xu Fu, Faruk Moonschi, Bernadeta R. Srijanto, Khaga Raj Neupane, Surya Aryal, Rosemary Calabro, Doo-Young Kim, C. Patrick Collier, Mustafa Habib Chowdhury, and Christopher I. Richards. Photoluminescence Enhancement, Blinking Suppression, and Improved Biexciton Quantum Yield of Single Quantum Dots in Zero Mode Waveguides. J. Phys. Chem. Lett. 0, 12, DOI: 10.1021/acs.jpclett.1c00450, (2021).

  21. K.R. Neupane, J.R. McCorkle, T.J. Kopper, J.E. Lakes, S.P. Aryal, M. Abdullah, et al., Macrophage-Engineered Vesicles for Therapeutic Delivery and Bidirectional Reprogramming of Immune Cell Polarization, ACS Omega, 2021, https://doi.org/10.1021/acsomega.0c05632

  22. Sandin, J. N., Aryal, S. P., Wilkop, T., Richards, C. I., Grady, M. E. Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells. J. Vis. Exp. (162), e61433, doi:10.3791/61433 (2020).

  23. Fu, X., Sompol, P., Brandon, J.A., Norris, C.M., Wilkop, T., Johnson, L.A., and Richards, C.I., In vivo single-molecule detection of nanoparticles for multiphoton fluorescence correlation spectroscopy to quantify cerebral blood flow, Nano Letters, 2020, https://doi.org/10.1021/acs.nanolett.0c02280

  24. Murach, K.A., Vechetti Jr., I.V., Van Pelt, D.W., Crow, S.E., Dungan, C.M., Figueiredo, V.C., Kosmac, K., Fu, X., Richards, C.I., Fry, C.S., McCarthy, J.J., Peterson, C.A., Fusion-independent satellite cell communication to muscle fibers during load-induced hypertrophy, Function, 2020, https://doi.org/10.1093/function/zqaa009

  25. Masud, A.A., Martin, W.E., Moonschi, F.H., Park, S.M., Srijanto, B.R., Graham, K.R., Collier, C.P., Richards, C.I., Mixed metal zero-mode guides (ZMWs) for tunable fluorescence enhancement, Nanoscale Advances, 2020, 2, 1894-1903.

  26. Fu, X., Song, Y., Masud, A., Nuti, K., DeRouchey, J.E., Richards, C.I., High-throughput fluorescence correlation spectroscopy enables analysis of surface components of cell-derived vesicles, Anal. Bioanal. Chem., 2020, 412(11): 2589-2597.

  27. Lakes, J.E., Richards, C.I. and Flythe, M.D., Inhibition of Bacteroidetes and Firmicutes by select phytochemicals, Anaerobe, 2019, 61:102145

  28. Chen, J., Wang, S., Zhang, Z., Richards, C. I. and Xu, R., Heat shock protein 47 (HSP47) binds to discoidin domain-containing receptor 2 (DDR2) and regulates its protein stability. J. Bio. Chem., 2019, 294(45):16846-16854

  29. Fu, X., Moonschi, F.H., Fox-Loe, A.M., Snell, A.A., Hopkins, D.M., Avelar, A.J., Henderson, B.J., Pauly, J.R., and Richards, C.I., Brain region specific single-molecule fluorescence imaging. Analytical Chemistry, 2019, 91, 10125-10131 

  30. Snell, A.A., Neupane, K.R., McCorkle, J.R., Fu, X., Moonschi, F.H., Caudill, E.B., Kolesar, J., and Richards, C.I., Cell-Derived Vesicles for In Vitro and In Vivo Targeted Therapeutic Delivery.  ACS Omega, 2019, 4, 12657-12664

  31. Song, Y., Fu, X., and Richards, C.I., A use case of humanware and cloud-based cyberinfrastructure: Time-series data classification using machine learning, PEARC-HARC, 2019, doi: 10.1145/3355738.3355748

  32. Zhang Z, Heidary DK, Richards CI. High resolution measurement of membrane receptor endocytosis. J Biol Methods 2018;5(4):e105. doi: 10.14440/jbm.2018.266

  33. Sun, Y., Heidary, D.K., Zhang, Z., Richards, C.I. & Glazer, E.C. Bacterial Cytological Profiling Reveals the Mechanism of Action of Anticancer Metal Complexes. Mol. Pharmaceutics, 2018, 15, 8, 3404-3416.

  34. Moonschi, F. H., Fox-Loe, A. M., Fu, X. and Richards, C. I. Mammalian Cell-derived Vesicles for the Isolation of Organelle Specific Transmembrane Proteins to Conduct Single Molecule Studies. Bio-protocol 2018, 8(9): e2825. DOI: 10.21769/BioProtoc.2825

  35. Moonschi, F.H., Hughes, C.B., Mussman, G.M. et al. Advances in micro- and nanotechnologies for the GLP-1-based therapy and imaging of pancreatic beta-cells, Acta Diabetol, 2018, 55(5): 405-418.

  36. Fox, A.M., Moonschi, F. H.; Richards, C. I.: Organelle-specific single-molecule imaging of α4β2 nicotinic receptors reveals the effect of nicotine on receptor assembly and cell-surface trafficking. J. Bio. Chem., 2017; 292(51):21159-21169.

  37. Zhang, Z.; Baksh, M.M.; Finn, M.G.; Heidary, D.K.; Richards, C.I., Direct Measurement of Trafficking of the Cystic Fibrosis Transmembrane Conductance Regulator to the Cell Surface and Binding to a Chemical Chaperone. Biochem. 2017, 240-249.

  38. Martin, W.E., Ge, N., Srijanto, B.R., Furnish, E., Collier, C.P., Trinkle, C., and Richards, C.I. Real time sensing of single ligand interactions with nanoaperture integrated microfluidic devices. ACS Omega, 2017, 2 (7), 3858

  39. Heidary, D., Loe, A.M., Richards, C.I., Glazer, E.C. A high-throughput screening assay using a photoconvertable protein for identifying inhibitors of transcription, translation, or proteasomal degradation. Slas. Disc., 2017 22, 399-407

  40. Loe, A.M., Henderson, B., and Richards, C.I. Utilizing pHluorin-tagged receptors to monitor subcellular localization and trafficking. J. Vis. Exp., 2017 121,

  41. Fox, A.M., Dwoskin, L.P., and Richards, C.I. Nicotinic Acetylcholine Receptors as Targets for Tobacco Cessation Therapeutics: Cutting-edge methodologies to understand receptor assembly and trafficking. Nicotinic Acetylcholine Receptor Technologies. NeuroMethods, 2016 117, 119-132

  42. Martin, W.E., Srijanto, B.R., Collier, C.P., Vosch, T., and Richards, C.I. A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides. J. Phys. Chem. A, 2016 120 (34), 6719-6727

  43. Fox, A.M.; Moonschi, F.H.; Richards, C.I. The nicotine metabolite, cotinine, alters the assembly and trafficking of a subset of nicotinic acetylcholine receptors. J. Bio. Chem., 2015, 290, 24403-24412.

  44. Das, S., Luk, C.M., Martin, W.E., Tang, L., Kim, D.Y., Lau, S.P., and Richards, C.I. Size and Dopant Dependent Single Particle Fluorescence Properties of Graphene Quantum Dots. J. of Phys. Chem. C, 2015, 119 (31).

  45. Wieskopf, J. S. et al.: The nicotinic alpha6 subunit gene determines variability in chronic pain sensitivity via cross-inhibition of P2X2/3 receptors. Sci Transl Med 2015, 7, 3009986.

  46. Moonschi FH, Effinger AK, Zhang X, Martin WE, Fox AM, Heidary DK, DeRouchey JE, Richards CI. Cell-Derived Vesicles for Single-Molecule Imaging of Membrane Proteins. Angew Chem Int Ed Engl 2015, 31(10):201408707.

  47. Srinivasan R., Henderson B.J., Lester H.A., Richards C.I. Pharmacological chaperoning of nAChRs: a therapeutic target for Parkinson's disease. Pharmacol Res 2014, 83, 20-29.

  48. Das S.K., Liu Y., Yeom S., Kim D.Y., Richards CI. Single-particle fluorescence intensity fluctuations of carbon nanodots. Nano Lett, 2014, 14:620-625.

  49. Nichols W.A., Henderson B.J., Yu C., Parker R.L., Richards C.I., Lester H.A., Miwa J.M.: Lynx1 Shifts alpha4beta2 Nicotinic Receptor Subunit Stoichiometry by Affecting Assembly in the Endoplasmic Reticulum. J. Bio. Chem. 2014, 5:573667

  50. Henderson B.J., Srinivasan R., Nichols W.A., Dilworth C.N., Gutierrez D.F., Mackey E.D.W., McKinney S., Drenan R.M., Richards C.I., Lester H.A. Nicotine exploits a COPI-mediated process for chaperone-mediated up-regulation of its receptors. J Gen Phys, 2013, 143:51-66, 2014.

  51. Srinivasan R., Richards C.I., Dilworth C., Moss F., Dougherty D., Lester H. Förster Resonance Energy Transfer (FRET) correlates of altered subunit stoichiometry in Cys-loop receptors, exemplified by nicotinic α4β2. Int J Mol Sci 13:10022-10040, 2012. PMCID: PMC3431844

  52. Richards C.I., Srinivasan R., Luong K., Srinivasan R., Turner S.W., Dougherty D.A., Korlach J., and Lester H.A. Live-cell imaging of single receptor composition using zero-mode waveguides. Nano Lett 12:3690-3694, 2012. PMCID: PMC3397148

  53. Srinivasan R., Richards C.I., Xiao C., Rhee D., Pantoja R., Mackey E.D.W., Miwa J., Lester H.A. Pharmacological chaperoning of nicotinic acetylcholine receptors inhibits the ER stress response. Mol Pharmacol 81:759-769, 2012. PMCID: PMC3362896

  54. Jablonski, A., Hsiang, J., Bagchi, P., Hull, N., Richards, C.I., Fahrni, C.J., and Dickson, R.M, Signal Discrimination Between Fluorescent Proteins in Live Cells by Long-wavelength Optical Modulation. J Phys. Chem. Lett. 2012, 3(23), 3585-3591.

  55. Richards C.I., Srinivasan R., Xiao C., Mackey E., Miwa J. and Lester H.A. Trafficking of α4* nicotinic receptors revealed by superecliptic phluorin: effects of a β4 ALS-associated mutation and chronic exposure to nicotine. J Biol Chem 287:286-298, 2011. PMCID: PMC3249079

  56. Richards C.I., Hsiang J.C., Khalil A., Hull N. and Dickson R.M. FRET-enabled optical modulation for high sensitivity fluorescence imaging. J Am Chem Soc 132:6318-6323, 2010. PMCID: PMC2874320

  57. Richards C.I., Hsiang J.C. and Dickson R.M. Synchronously amplified fluorescence image recovery (SAFIRe). J Phys Chem B 114:660-665, 2010. PMCID: PMC2804033

  58. Patel, S.A., M. Cozzuol J.M. Hales, C.I. Richards, M. Sartin, J.C. Hsiang, T. Vosch, J.W. Perry, R.M. Dickson, “Electron Transfer-Induced Blinking in Ag Nanodot Fluorescence”, J. Phys. Chem. C, 2009. 113(47)

  59. Richards, C.I., D. Senapati, J.C. Hsiang, S.A. Patel, T. Vosch, and R.M. Dickson, “Optically Modulated Fluorophores for Selective Fluorescence Signal Recovery”, J. Amer. Chem. Soc., 2009. 131(13)

  60. Richards, C.I., S. Choi, Hsiang, J.C., Antoku, Y., Vosch, T., Bongiorno, A., Tzeng, Y.L., and Dickson, R.M. “Oligonucleotide-stabilized Ag nanocluster fluorophores”, J. Amer. Chem. Soc., 2008. 130(15)

  61. Patel, S.A., Richards, C.I., Hsiang, J.C., and Dickson, R.M. “Water-soluble Ag nanoclusters exhibit strong two-photon-induced fluorescence”, J. Amer. Chem. Soc., 2008. 130(35)

  62. Yu, J., Choi, S., Richards, C.I., Anotku, Y., and Dickson, R.M. "Live Cell Surface Labeling with Fluorescent Ag Nanocluster Conjugate" Photochem. and Photobio., 2008

  63. Vosch, T., Antoku, Y., Hsiang, J.C., Richards, C.I., Gonzalez, J.I., and Dickson, R.M. “Strongly emissive individual DNA-encapsulated Ag nanoclusters as single-molecule fluorophores”, PNAS, 2007. 104(31)